[1]刘野,李应东.当归红芪超滤膜提取物含药血清对辐射心肌成纤维细胞SOD和羟脯氨酸的影响[J].甘肃中医药大学学报,2010,(03):1-3.
 Liu Ye,Li Yingdong.Effects of serum containing gradocol membranes extract from Angelica Sinensis and Radix Hedysari on superoxide dismutase and oxyproline hydroxyproline of radiation cardiac fibroblasts[J].Journal of Gansu University of Chinese Medicine,2010,(03):1-3.
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当归红芪超滤膜提取物含药血清对辐射心肌成纤维细胞SOD和羟脯氨酸的影响()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
期数:
2010年03期
页码:
1-3
栏目:
实验研究
出版日期:
1900-01-01

文章信息/Info

Title:
Effects of serum containing gradocol membranes extract from Angelica Sinensis and Radix Hedysari on superoxide dismutase and oxyproline hydroxyproline of radiation cardiac fibroblasts
作者:
刘野 李应东
甘肃中医学院, 甘肃 兰州 730000
Author(s):
Liu Ye Li Yingdong
Gansu College of TCM, Lanzhou 730000, China
关键词:
当归红芪超滤膜提取物心肌成纤维细胞辐射SOD羟脯氨酸
Keywords:
Angelica SinensisRadix Hedysarigradocol membranes extractcardiac fibroblastradiationsuperoxide dismutaseoxyproline hydroxyproline
分类号:
R285.5
摘要:
目的 观察当归红芪超滤膜提取物含药血清对辐射心肌成纤维细胞SOD和羟脯氨酸的影响。方法 用胰酶-胶原酶消化法分离培养乳鼠心肌成纤维细胞,建立体外培养体系,通过10万分子量的超滤膜提取物制备不同浓度和不同期限的含药血清。将已经过药物干预24 h的第2代细胞进行辐射剂量为10 Gy的辐射,继续培养24 h,黄嘌呤氧化法检测超氧化物歧化酶(SOD)活性,碱水解法检测羟脯氨酸含量。结果 与空白组比较,模型组SOD活性显著下降,羟脯氨酸含量显著增加(P<0.01);与模型组比较,药物组SOD活性显著升高(P<0.05或P<0.01),且药物组(7 d)显著优于药物组(3 d)(P<0.05),而羟脯氨酸含量下降并不显著(P>0.05)。结论 辐射可引起SOD活性下降、羟脯氨酸含量增加,而当归红芪超滤膜提取物能去除氧自由基,并且长期疗效优于短期,但对羟脯氨酸含量并无影响。
Abstract:
Objective To observe the effects of serum containing gradocol membranes extract from Angelica Sinensis(AS) and Radix Hedysari (RH) on superoxide dismutase (SOD) and oxyproline hydroxyproline(Hyp) of radiation cardiac fibroblasts.Methods Cardiac fibroblasts (CFs) of neonatal rats were isolated and cultured by digestive method of pancreatin and collagenase, and the cell culture systems were established in vitro. Production of different concentrations and different time of drug-containing serum was be obtained by gradocol membranes extract from AS and RH with 100 000 molecular weight.The second passages of cardiac fibroblasts that were intervened by drug for 24 hours were given radiation of 10 Gy X-ray,and continued to be cultured for 24 hours,and measured SOD and Hyp by xanthine oxidase method and basic hydrolysis method.Results Compared with blank group, the activity of SOD of model group was obviously decreased,and the content of Hyp was obviously increased(P<0.01). Compared with model group, the activity of SOD of drug group was obviously increased(P<0.05 or P<0.01),and drug group of 7 days was better than 3 days (P<0.05),but the content of Hyp wasn’t obviously decreased(P>0.05).Conclusion The radiation can cause the activity of SOD to decrease and the content of Hyp to increase. Gradocol membranes extract from AS and RH can remove oxygen free radicals, and the long-term efficacy was better than the short-term,but it isn’t effect on the content of Hyp.

参考文献/References:

[1] 徐府奇,王琛,谢红.低剂量辐射预处理对放射性脑损伤自由基的影响[J].苏州大学学报(医学版),2006,26 (6):951.
[2] Siesjo B X,Zhal Q,Pahlmark K,et al. Glutamute,calcium and free radicals as mediators of ischemia brain damage[J]. Amm Thorac Surg,1995,59:1316.
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备注/Memo

备注/Memo:
收稿日期:2010-03-15;改回日期:2010-03-20。
基金项目:十一五国家科技支撑计划项目子课题(2006BAI06A20-04)
作者简介:刘野(1981-),男,在读硕士研究生。研究方向:中西医结合治疗心血管疾病。
更新日期/Last Update: 1900-01-01