[1]汪继峰,孙岩,杨玉华.高效液相色谱法测定复方平喘胶囊中槲皮素的含量[J].甘肃中医药大学学报,2012,(04):61-63.
 Wang Jifeng,Sun Yan,Yang Yuhua.Determination of the content of quercetin in Fufang Pingchuan Capsule by HPLC[J].Journal of Gansu University of Chinese Medicine,2012,(04):61-63.
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高效液相色谱法测定复方平喘胶囊中槲皮素的含量()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
期数:
2012年04期
页码:
61-63
栏目:
技术与方法
出版日期:
1900-01-01

文章信息/Info

Title:
Determination of the content of quercetin in Fufang Pingchuan Capsule by HPLC
作者:
汪继峰 孙岩 杨玉华
兰州佛慈制药股份有限公司, 甘肃 兰州 730046
Author(s):
Wang Jifeng Sun Yan Yang Yuhua
Lanzhou Foci Company Limited, Lanzhou 730046, China
关键词:
复方平喘胶囊槲皮素高效液相色谱法含量测定
Keywords:
Fufang Pingchuan Capsuleoleanolic acidHPLCcontent determination
分类号:
R284.2
摘要:
目的 建立复方平喘胶囊中槲皮素含量的测定方法。方法 采用Phenomenex DDS-C18色谱柱(5 μm,4.6 mm×250 mm),流动相为乙腈-0.1%磷酸(36:64),检测波长为370 nm,柱温40℃,流速0.6 mL/min。结果 槲皮素在0.1184~0.7104 μg范围内线性关系良好,r=0.9998,平均回收率为97.7%,样品中槲皮素含量为7.01 mg/g。结论 高效液相色谱(HPLC)法准确可靠、精密度高、重现性好,可作为复方平喘胶囊中槲皮素含量的测定方法。
Abstract:
Objective To establish the method on determination of the content of quercetin in Fufang Pingchuan Capsule.Methods Adopted HPLC method, chromatographic conditions were available as follows Phenomenex DDS-C18 column(5 μm,4.6 mm×250 mm), acetonitril and 0.1% phosphoric acid (36:64) as mobile phase, detecting wavelength was 370 nm, temperature of the column was 40℃, the flowrate was 0.6 mL/min.Results The linear correlation of quercetin was well within 0.1184~0.7104 μg(r=0.9998). The average recovery rate was 97.7%,the content of quercetin in sample was 7.01 mg/g.Conclusion HPLC method is accurate, sensitive and reproducible,it can be used as the determination method of the content of quercetin in Fufang Pingchuan Capsule.

参考文献/References:

[1] 周旭美,蓝英妮.HPLC法测定对坐叶片中槲皮素的含量[J].中国药房,2008,19(24):1890-1892.
[2] 潘馨.RP-HPLC测定几种闽产杜鹃中槲皮素的含量[J].药物分析杂志,2002,22(6):436.

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备注/Memo

备注/Memo:
收稿日期:2012-04-19;改回日期:2012-05-14。
作者简介:汪继峰(1974-),男,工程师,医学学士。研究方向:中药制剂及质量研究。
更新日期/Last Update: 1900-01-01