[1]林瑞珠,许建峰,朱宁,等.回医烙灸疗法对实验性兔膝骨性关节炎软骨细胞凋亡的影响[J].甘肃中医药大学学报,2014,31(01):1-3.
 Lin Ruizhu,Xu Jianfeng,Zhu Ning,et al.The effect of moxibustion in Huiyi on articular chondrocyte apoptosis of osteoarthritis rabbits[J].Journal of Gansu University of Chinese Medicine,2014,31(01):1-3.
点击复制

回医烙灸疗法对实验性兔膝骨性关节炎软骨细胞凋亡的影响()
分享到:

《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
31卷
期数:
2014年01期
页码:
1-3
栏目:
实验研究
出版日期:
2014-02-25

文章信息/Info

Title:
The effect of moxibustion in Huiyi on articular chondrocyte apoptosis of osteoarthritis rabbits
作者:
林瑞珠 许建峰 朱宁 牛子瞻 席朝垒
宁夏医科大学总医院, 宁夏 银川 750004
Author(s):
Lin Ruizhu Xu Jianfeng Zhu Ning Niu Zizhan Xi Chaolei
General Hospital of Ningxia Medical University, Yinchuan 750004, China
关键词:
膝骨性关节炎回医烙灸疗法软骨细胞凋亡形态学TUNEL法
Keywords:
knee osteoarthritismoxibustion in Huiyichondrocyte apoptosismorphologyTUNEL method
分类号:
R245.8
摘要:
目的 观察回医烙灸疗法对兔膝骨性关节炎软骨细胞凋亡的影响。方法 将30只新西兰兔随机分为正常对照组、模型组和回医烙灸组,每组10只。采用石膏固定的方法制作兔左膝骨性关节炎模型。造模6周后,回医烙灸组在膝关节局部行回医烙灸疗法,每2 d 1次,每次20 min,治疗4周;正常对照组和模型组不予干预。治疗4周后处死3组动物,取胫骨平台和股骨内侧髁软骨,进行标本的形态学观测,末端标记(TUNEL)法检测软骨细胞凋亡情况。结果 模型组TUNEL阳性软骨细胞散在于软骨全层,且数量多,凋亡指数显著高于正常对照组,2组比较差异有统计意义(P<0.05);回医烙灸组表层也有TUNEL阳性软骨细胞,但凋亡指数显著低于模型组,2组比较差异有统计意义(P<0.05)。结论 回医烙灸疗法可以减少兔膝骨性关节炎软骨细胞的凋亡,从而延缓膝骨性关节炎的病情进展。
Abstract:
Objective To observe the effect of moxibustion in Huiyi on articular chondrocyte apoptosis of osteoarthritis rabbits.Methods A total of 30 adult New Zealand rabbits were randomly divided into normal group, model group and moxibustion group. Each group was 10 rabbits. The experimental knee osteoarthritis model was constructed by plaster fixation.The animals of moxibustion group were treated with moxibustion from 6 weeks to 10 weeks,then all the rabbits were executed. The changes of chondrocyte were observed by HE dyeing.The chondrocyte apoptosis was detected by TUNEL.Results The chondrocyte apoptosis change in normal group and moxibustion group were better than one in the model group (P<0.05).Conclusion Moxibustion in Huiyi can control the expression of chondrocyte apoptosis in the experimental knee osteoarthritis model. It maybe delays and inhibits the degeneration of chondrocyte.

参考文献/References:

[1] 施桂英.关节炎概要[M].北京:中国医药科技出版社,2000:339-342.
[2] 许建峰,林瑞珠,牛子瞻,等.回医烙灸疗法对退变腰椎间盘中基质MMP-3表达的影响[J].福建中医药,2013,44(2):53-54.
[3] 汪青春.骨与关节炎动物模型的建立及选择[J].中医正骨,1998,10(3):152.
[4] 严雪港,鲍同柱.骨关节炎软骨细胞凋亡研究进展[J].陕西医学杂志,2010,39(3):354-356.
[5] 魏亮.骨关节炎软骨病因及临床研究进展[J].河北医药,2010,32(5):611-614.
[6] 宋岘.回回药方考释[M].北京:中华书局,2000:471-476.
[7] 杨桦.《回回药方》中的灸法特色[J].宁夏医科大学学报,2011,33(6):501-502.
[8] 乔建荣,粱颖,靳萱,等.《回回药方》烙灸疗法探析[J].宁夏医科大学学报,2011,33(2):101-102.

相似文献/References:

[1]俞斌,姚新苗.针刀微创松解结合中药内服治疗膝骨性关节炎46例临床观察[J].甘肃中医药大学学报,2016,33(03):83.[doi:10.16841/j.issn1003-8450.2016.03.27]
[2]王雷,杨利学,张效科.中西医结合治疗膝关节骨性关节炎106例疗效观察[J].甘肃中医药大学学报,2006,(05):29.
[3]张学良,宋敏,宋贵杰.三步疗法治疗膝骨性关节炎疗效观察[J].甘肃中医药大学学报,2007,(02):34.
[4]王钢,李宁,王佳.中西医结合治疗膝骨性关节炎疗效观察[J].甘肃中医药大学学报,2009,(04):29.
[5]张长彪,关新红.活血通络丸治疗膝骨性关节炎66例[J].甘肃中医药大学学报,2010,(04):43.
[6]黄桂林,史国号,王少波.温针灸加中药贴敷联合玻璃酸钠注射治疗膝骨性关节炎65例疗效观察[J].甘肃中医药大学学报,2012,(02):40.
[7]乔斌,李盛华,周明旺,等.中医药对膝骨性关节炎的作用机制及治疗研究进展[J].甘肃中医药大学学报,2014,31(04):76.
[8]宋敏,张晓刚,刘建军,等.透明质酸钠治疗膝骨性关节炎[J].甘肃中医药大学学报,2001,(03):25.
[9]魏兵,刘国雄,全健.外敷中药膏联合关节腔内注射玻璃酸钠治疗膝骨性关节炎50例临床观察[J].甘肃中医药大学学报,2016,33(05):44.[doi:10.16841/j.issn1003-8450.2016.05.13]
 WEI Bing,LIU Guoxiong,QUAN Jian.Clinical observation on external application of traditional Chinese ointments plus sodium hyaluronate intra-articular injection in treating 50 cases of knee osteoarthritis[J].Journal of Gansu University of Chinese Medicine,2016,33(01):44.[doi:10.16841/j.issn1003-8450.2016.05.13]
[10]李民,贺鑫,林哲辉,等.疯痹贴治疗膝骨性关节炎风寒湿痹证临床观察[J].甘肃中医药大学学报,2016,33(06):62.[doi:10.16841/j.issn1003-8450.2016.06.19]
 LI Min,HE Xin,LIN Zhehui,et al.Clinical observation of curative effect on knee osteoarthritis treated with Fengbi Tie[J].Journal of Gansu University of Chinese Medicine,2016,33(01):62.[doi:10.16841/j.issn1003-8450.2016.06.19]

备注/Memo

备注/Memo:
收稿日期:2013-09-03
基金项目:宁夏科技攻关项目(2012036)
作者简介:林瑞珠(1979-),女,主治医师。研究方向:回族医药和针灸推拿治疗退行性脊柱关节病的临床及基础研究。
更新日期/Last Update: 1900-01-01