[1]刘娟娟,刘雪枫,陈晖,等.反相高效液相色谱法测定藏药薰倒牛中木犀草素及槲皮素含量[J].甘肃中医药大学学报,2015,32(06):23-25.
 LIU Juanjuan,LIU Xuefeng,CHEN Hui,et al.Determination of luteolin and quercetin in Biebersteinia heterostemon Maxim. by RP-HPLC[J].Journal of Gansu University of Chinese Medicine,2015,32(06):23-25.
点击复制

反相高效液相色谱法测定藏药薰倒牛中木犀草素及槲皮素含量()
分享到:

《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
32卷
期数:
2015年06期
页码:
23-25
栏目:
技术与方法
出版日期:
2015-12-25

文章信息/Info

Title:
Determination of luteolin and quercetin in Biebersteinia heterostemon Maxim. by RP-HPLC
作者:
刘娟娟1 刘雪枫1 陈晖2 赵慧巧1 卢年华1 景明1
1. 甘肃中医药大学药学院, 甘肃兰州 730000;
2. 甘肃中医药大学理科教学部, 甘肃兰州 730000
Author(s):
LIU Juanjuan1 LIU Xuefeng1 CHEN Hui2 ZHAO Huiqiao1 LU Nianhua1 JING Ming1
1. Pharmacy School of Gansu University of Chinese Medicine, Lanzhou 730000, China;
2. Science Teaching Department of Gansu University of Chinese Medicine, Lanzhou 730000, China
关键词:
藏药熏倒牛木犀草素槲皮素反相高效液相色谱含量测定
Keywords:
Biebersteinia heterostemon Maxim.luteolinquercetinRP-HPLCassaying
分类号:
R284.2
摘要:
目的建立藏药熏倒牛中木犀草素及槲皮素的含量测定方法。方法采用反相高效液相色谱(RP-HPLC)法,以SB-C18为填充剂,甲醇-0.4%磷酸水溶液(48:52)为流动相,检测波长为350 nm,建立了一种可同时测定藏药熏倒牛药材中木犀草素与槲皮素含量的方法。结果木犀草素回归方程为Y=8.97×107X +1.06×105(R=0.9999,n=5),线性范围为6.08~36.48μg/mL,平均加样回收率为97.69%,RSD为1.56%;槲皮素回归方程为Y=6.91×107X+7.77×103(R=0.9998,n=5),线性范围为2.56~19.2μg/mL,平均加样回收率为98.31%,RSD为1.41%。结论该方法简便、快速、准确,可用于同时测定熏倒牛中木犀草素、槲皮素的含量及药材质量控制。
Abstract:
Objective To establish a method for determining the content of luteolin and quercetin in Bieberstei-nia heterostemon Maxim.(traditional Tibetan medicine). Methods The content of luteolin and quercetin in Biebersteinia heterostemon Maxim. was determined by RP-HPLC. The mobile phase was methanol:phosphoric acid solution (0.4%) at 48:52,and UV detection wavelength was set at 350 nm. A method was established for simultaneously determining the content of luteolin and quercetin in Biebersteinia heterostemon Maxim.. Results The equation of linear regression of luteolin was Y=8.97×107X +1.06×105(R=0.9999,n=5),the linear range was 6.08 to 36.48μg/mL,the average sample recovery rate was 97.69%,RSD was 1.56%. The equation of linear regression of quercetin was Y=6.91×107X +7.77×103(R=0.9998,n=5),the linear range was 2.56 to 19.2μg/mL. The ave-rage sample recovery rate was 98.31%,RSD was 1.41%. Conclusion The method is simple,rapid and accurate,which can determine the content of luteolin and quercetin in Biebersteinia heterostemon Maxim. simultaneously and control medicinal materials quality.

参考文献/References:

[1] 杨永昌.藏药志[M].西宁:青海人民出版社,1991:263.
[2] 中国科学院中国植物志编辑委员会.中国植物志(第一卷)[M].北京:科学出版社,2004:415.
[3] 张晓峰,胡伯林,周炳南.藏药薰倒牛的活性物质研究[J].药学学报,1995,9(3):211-214.
[4] 王晶晶,景明,李炀,等.藏药熏倒牛质量标准研究[J].中药研究与开发,2013,29(4):48-49.
[5] 王秋玲.HPLC测定独一味中木犀草素的含量[J].微量元素与健康研究,2006,23(2):43-44.
[6] 陈晖,戴红霞.RP-HPLC测定藏药薰倒牛中木犀草素的含量[C].兰州:甘肃省化学会第二十八届年会暨第十届中学化学教学经验交流会,2013:3.

相似文献/References:

[1]赵慧巧,陈晖,刘雪枫,等.复方湿生扁蕾胶囊不同配伍对湿生扁蕾化学成分含量的影响[J].甘肃中医药大学学报,2015,32(04):24.
 ZHAO Huiqiao,CHEN Hui,LIU Xuefeng,et al.Influence of different compatibility in Compound Shishengbianlei Capsule on the content of chemical components of Gentianopsis paludosa[J].Journal of Gansu University of Chinese Medicine,2015,32(06):24.

备注/Memo

备注/Memo:
收稿日期:2015-02-02
基金项目:兰州市社会发展科技计划项目(2012-1-57)。
作者简介:刘娟娟(1989-),女,在读硕士研究生。研究方向:中藏药新制剂的开发研究。
通信作者:景明(1963-),男,教授,博士生导师,理学学士。研究方向:中藏药新制剂的开发研究。E-mail:jm@gszy.edu.cn
更新日期/Last Update: 1900-01-01