[1]牛亭惠,吴国泰,陈振鹤,等.水通道蛋白3在慢传输型便秘模型大鼠结肠黏膜中的表达[J].甘肃中医药大学学报,2017,34(02):7-10.[doi:10.16841/j.issn1003-8450.2017.02.02]
 NIU Tinghui,WU Guotai,CHEN Zhenhe,et al.Expression of aquaporin-3 in colon mucosa of rats with slow transit constipation[J].Journal of Gansu University of Chinese Medicine,2017,34(02):7-10.[doi:10.16841/j.issn1003-8450.2017.02.02]
点击复制

水通道蛋白3在慢传输型便秘模型大鼠结肠黏膜中的表达
分享到:

《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
34卷
期数:
2017年02期
页码:
7-10
栏目:
实验研究
出版日期:
2017-04-25

文章信息/Info

Title:
Expression of aquaporin-3 in colon mucosa of rats with slow transit constipation
作者:
牛亭惠1 吴国泰12 陈振鹤1 杜丽东12 任远12
1. 甘肃中医药大学药学院, 甘肃 兰州 730000;
2. 甘肃省中药药理与毒理学重点实验室, 甘肃 兰州 730000
Author(s):
NIU Tinghui1 WU Guotai12 CHEN Zhenhe1 DU Lidong12 REN Yuan12
1. Pharmacy College, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730000, China;
2. Key Laboratory for TCM Pharmacology and Toxicology in Gansu Province, Lanzhou, Gansu, 730000, China
关键词:
慢传输型便秘结肠黏膜水通道蛋白3大鼠免疫组化
Keywords:
slow transit constipationcolon mucosaaquaporin-3ratsimmunohistochemistry
分类号:
R-332
DOI:
10.16841/j.issn1003-8450.2017.02.02
摘要:
目的 通过复制大鼠慢传输型便秘(STC)模型,观察水通道蛋白3(AQP3)在模型大鼠结肠黏膜中的表达,进而探讨其与STC的关系。方法 将40只SD大鼠随机分为空白组和模型组,各20只。模型组灌胃盐酸洛哌丁胺10 mg/kg,空白组灌胃等容量SPF级实验动物饮用水,2组均1次/d,连续灌胃42 d。实验期间各组大鼠自由进食、饮水,观察大鼠的一般状态并检测体质量变化;造模第40天检测首粒黑便排出时间;第42天计算小肠推进率,并对结肠组织进行免疫组化染色,显微镜下观察AQP3阳性染色情况,计算平均积分光密度(IOD)、平均光密度(OD)及模型组的增加率。结果 造模第4,5,6周,与空白组比较,模型组大鼠体质量增长均显著减慢(P<0.05或P<0.01)。模型组第4周左右开始出现固体硬质粪便,粪便成球形、锥形或串珠样,质硬、表面少有光泽。首粒黑便排出时间显著延长(P<0.05或P<0.01),但小肠推进率与空白组比较差异无统计意义(P>0.05)。模型组大鼠结肠黏膜吸收细胞和杯状细胞呈棕褐色,染色明显;模型组大鼠AQP3表达水平显著增加,OD和IOD增加率分别为11.1%,118.5%,与空白组比较,差异均有高度统计意义(P<0.01)。结论 STC模型大鼠近端结肠黏膜上都有AQP3的表达,且可能在STC的发生、发展中起一定的作用。
Abstract:
Objective To observe the expression of aquaporin-3 in colon mucosa of model rats with slow transit constipation (STC) and explore the correlation. Methods 40 SD rats were randomly divided into control group and model group,20 cases each group. The model group was given loperammide hydrochloride (10 mg/kg) by gavage;the control group was perfused with the same dosage of SPF laboratory animal drinking water;once a day,la-sting for 42 days. General condition and body weight were observed and measured. On 40th day,the first defecations time was recorded;on 42nd,small intestinal propulsion rates were measured. The expression of aquaporin-3 was observed through immunohisto-chemical staining method. IOD,OD and increasing rate were calculated. Results On 4th,5th and 6th week,compared with control group,increase of body weight in model group slowed down obviously (P<0.05 or P<0.01). On 4th week,rats in model group defecated hard and dark faeces in spherical,cone or beads-on-string shape. The first defecations time was significantly extended (P<0.05 or P<0.01). As to intestine advance rate,there was no any significant difference between two groups (P>0.05). Absorptive cells and goblet cells in colon mucosa were stained in brown. The levels of aquaporin-3 expression of model rats increased. Increa-sing rates of IOD and OD were 11.1% and 118.5% respectively. Compared with control group,the difference was significant (P<0.01). Conclusion The expression of aquaporin-3 in proximal colon mucosa may play an important role in the incidence and development of STC.

参考文献/References:

[1] 刘井如,季宇彬,陈明苍.便秘动物模型的研究进展[J].中国实验方剂学杂志,2012,18(22):353.
[2] 刘仍海.通调三焦法治疗泻药性便秘的临床与实验研究[D].北京:北京中医药大学,2006.
[3] 刘海峰,何俊堂,汪兴伟,等.大鼠慢传输型便秘模型的建立及其结肠肌电变化检测[J].武警医学,2004,12(3):887-888.
[4] 刘影.菝葜对便秘小鼠肠动力影响的实验研究[D].哈尔滨:黑龙江中医药大学,2011.
[5] 吴霜,程滢瑞,周锦勇,等.洛哌丁胺构建大鼠便秘模型对水通道蛋白AQP3和AQP8的影响[J].世界华人消化杂志,2014,22(7):969-974.
[6] 麦玉书.便秘的现代中医文献整理及循证医学研究[D].广州:广州中医药大学,2011.
[7] 李立胜,王俊平.洛哌丁胺对腹泻模型大鼠结肠水通道蛋白4表达的影响[J].胃肠病学和肝病学杂志,2013,22(12):1 227.
[8] 涂星,柴玉娜,唐洪梅,等.肝郁脾虚型肠易激综合征便秘大鼠模型的建立和评价[J].中国实验动物学报,2015,23(1):34.
[9] Magn F,Sarto C,Ticozzi D,et al.Proteomic knowledge of human aquaporins[J].Proteomics,2006,6(20):5 637-5 649.
[10] Ikarashi N,Kon R,Iizasa T.Inhibition of aquaporin-3 water channel in the colon induces diarrhea[J].Biol Pharm Bull,2012,35(6):957-962.
[11] 骆汝涛.阿托品对便秘大鼠结肠AQP3表达的影响[D].南充:川北医学院,2012.
[12] 智会,袁维堂.水通道蛋白3,4,8在大鼠慢传输型便秘模型结肠黏膜中的表达[J].中华胃肠外科杂志,2011,14(6):459-461.

相似文献/References:

[1]王晓龙,周仲瑜.穴位埋线治疗慢传输型便秘30例疗效观察[J].甘肃中医药大学学报,2014,31(05):60.
 Wang Xiaolong,Zhou Zhongyu.Clinical observation of acupoint catgut-embedding therapy in treating 30 cases with slow transit constipation[J].Journal of Gansu University of Chinese Medicine,2014,31(02):60.

备注/Memo

备注/Memo:
收稿日期:2016-09-28
基金项目:2015年甘肃省“陇原青年创新人才扶持计划”项目(2015-003);甘肃省中药现代制药工程研究院基金项目(YWW-2015050)
作者简介:牛亭惠(1990-),女,在读硕士研究生。研究方向:中药药理与毒理。
通信作者:任远(1963-),男,教授,博士生导师,医学学士,主要从事中药药理、毒理学教学与科研工作。E-mail:ry@gszy.edu.cn
更新日期/Last Update: 2017-07-11