[1]杨意,朱向东,李婷,等.四神丸对溃疡性结肠炎大鼠血清相关炎性因子表达及结肠组织病理形态的影响[J].甘肃中医药大学学报,2017,34(02):22-26.[doi:10.16841/j.issn1003-8450.2017.02.05]
 YANG Yi,ZHU Xiangdong,LI Ting,et al.Effects of Sishen Wan on the expression of associated inflammatory factors of serum in rats with ulcerative colitis[J].Journal of Gansu University of Chinese Medicine,2017,34(02):22-26.[doi:10.16841/j.issn1003-8450.2017.02.05]
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四神丸对溃疡性结肠炎大鼠血清相关炎性因子表达及结肠组织病理形态的影响
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
34卷
期数:
2017年02期
页码:
22-26
栏目:
实验研究
出版日期:
2017-04-25

文章信息/Info

Title:
Effects of Sishen Wan on the expression of associated inflammatory factors of serum in rats with ulcerative colitis
作者:
杨意 朱向东 李婷 翟艳会
甘肃中医药大学基础医学院, 甘肃 兰州 730000
Author(s):
YANG Yi ZHU Xiangdong LI Ting ZHAI Yanhui
Basic Medical College, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730000, China
关键词:
溃疡性结肠炎四神丸白细胞介素-2肿瘤坏死因子-α细胞信号转导抑制因子3大鼠实验研究
Keywords:
ulcerative colitisSishen WanIL-2TNF-αSOCS3ratsexperimental study
分类号:
R285.5
DOI:
10.16841/j.issn1003-8450.2017.02.05
摘要:
目的 研究四神丸对溃疡性结肠炎(UC)大鼠血清白细胞介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)和细胞信号转导抑制因子3(SOCS3)表达及结肠组织病理形态的影响。方法 将SPF级Wistar大鼠随机分为空白组、模型组、美沙拉嗪组及四神丸组4组,采用复合方法复制脾肾阳虚型UC大鼠模型。各组大鼠以相关药物及生理盐水灌胃21 d后处死,取大鼠结肠,肉眼观察结肠黏膜大体形态损伤并进行评分;分离各组大鼠血清,采用ELISA法检测血清IL-2,TNF-α,SOCS3的表达水平。结果 与空白组比较,模型组大鼠结肠黏膜大体形态损伤评分及血清IL-2,TNF-α水平显著升高,血清SOCS3水平显著降低,差异均有统计意义(P<0.05);与模型组比较,美沙拉嗪组与四神丸组大鼠结肠黏膜大体形态损伤评分及血清TNF-α水平显著降低,血清SOCS3水平显著升高,差异均有统计意义(P<0.05)。结论 四神丸可能通过调节血清TNF-α,SOCS3的水平来调控肠上皮免疫系统,从而达到治疗UC的目的。
Abstract:
Objective To explore the effects of Sishen Wan on the expression of serum hypothalamus cytokine interleukin 2 (IL-2),tumor necrosis factor-α (TNF-α) and suppressor of cytokine signaling 3 (SOCS3) as well as pathological forms of colonic tissue in model rats with ulcerative colitis (UC). Methods SPF Wistar rats were randomly divided into control group,model group,mesalazine group and Sishen Wan group. UC rat models were reproduced by complex method. All rats were given drugs and saline by gavage for 21 days. Then microscopic da-mage on colonic mucosa was observed. The levels of IL-2,TNF-α and SOCS3 in serum were detected by ELISA. Results Compared with control group,the macroscopic damage scores,the levels of serum IL-2 and TNF-α in model group increased,while SOCS3 decreased obviously(P<0.05); compared with model group,the macroscopic damage scores and the levels of serum TNF-α decreased,while SOCS3 increased in mesalazine group and Sishen Wan Group. The differences were all significant (P<0.05). Conclusion Sishen Wan can cure UC by adjusting the levels of TNF-α and SOCS3 factors to regulate and control the immune system of intestinal epithelium.

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备注/Memo

备注/Memo:
收稿日期:2016-09-15
作者简介:杨意(1990-),男,在读硕士研究生。研究方向:中医治则、治法及其临床应用研究。
更新日期/Last Update: 2017-07-11