[1]张强,冯华,王世俊,等.高效液相色谱法测定妇科止血灵中五味子醇甲的含量[J].甘肃中医药大学学报,2017,34(02):50-52.[doi:10.16841/j.issn1003-8450.2017.02.15]
 ZHANG Qiang,FENG Hua,WNAG Shijun,et al.Content determination of schisandrin in Fuke Zhixueling by HPLC[J].Journal of Gansu University of Chinese Medicine,2017,34(02):50-52.[doi:10.16841/j.issn1003-8450.2017.02.15]
点击复制

高效液相色谱法测定妇科止血灵中五味子醇甲的含量
分享到:

《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
34卷
期数:
2017年02期
页码:
50-52
栏目:
技术与方法
出版日期:
2017-04-25

文章信息/Info

Title:
Content determination of schisandrin in Fuke Zhixueling by HPLC
作者:
张强 冯华 王世俊 郑皇雅
遵义市食品药品检验所中药室, 贵州 遵义 563002
Author(s):
ZHANG Qiang FENG Hua WNAG Shijun ZHENG Huangya
TCM Office, Zunyi Institute for Food & Drug Control, Zunyi, Guizhou, 563002, China
关键词:
妇科止血灵五味子醇甲高效液相色谱含量测定
Keywords:
Fuke ZhixuelingschisandrinHPLCcontent determination
分类号:
R284.2
DOI:
10.16841/j.issn1003-8450.2017.02.15
摘要:
目的 制定妇科止血灵中五味子醇甲的含量测定方法。方法 采用高效液相色谱(HPLC)法测定妇科止血灵中五味子醇甲的含量。色谱柱为Agilent C18(5 μm,250 mm×4.6 mm),流动相为甲醇-水(65:35),流速为1.0 mL/min,检测波长为250 nm,柱温为25℃;并对线性关系、精密度、稳定性、重复性等进行方法学考察。结果 五味子醇甲在5.843~52.587 μg/mL范围内呈良好的线性关系,回归方程为Y=2.453 9X+3.076 4,r=0.999 9;本品的平均回收率为102.02%,RSD为1.3%;五味子醇甲平均含量为59.40 μg/g。结论 该方法简便、灵敏、准确、重现性好、精密度高,可作为妇科止血灵中五味子醇甲的定量测定方法。
Abstract:
Objective To establish content test methods for schisandrin in Fuke Zhixueling. Methods Content of schisandrin in Fuke Zhixueling was determined by HPLC. The samples were separated on the column of Agilent C18(5 μm,250 mm×4.6 mm),which moving phase was methanol and water (65:35). The flow rate was 1.0 mL/min. The detected wave-length was at 250 nm. The column temperature was at 25℃. Methodology validation was used to test linearity,precision,stability,repeatability. Results The regression equation of schisandrin was Y=2.453 9X+3.076 4,r=0.999 9,showing a good linearity in the range of 5.843 to 52.587 μg/mL. The average recovery rate was 102.02% and RSD was 1.3%. The average content of schisandrin was 59.40 μg/g. Conclusion The method is simple,sensitive,accurate and with good reproducibility,high accuracy,which can be used to quantitatively determine the schisandrin in Fuke Zhixueling.

参考文献/References:

[1] 国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:61.
[2] 罗集鹏.生药学[M].北京:中国医药科技出版社,2004:241.
[3] 施祖勇,谭军.高效液相色谱法测定复方益肝灵片中五味子醇甲、五味子醇乙和五味子甲素的含量[J].中南药学,2011,9(4):264-267.
[4] 谭晓虹,田嘉铭,王治宝,等.HPLC法测定南五味子中五味子醇甲和五味子酯甲的含量[J].河北北方学院学报(自然科学版),2011,27(6):70-72.
[5] 吴晓凤,陈虹,赵志茹.HPLC法测定生脉饮中五味子醇甲的含量[J].中国中药杂志,2003,28(1):43-44.
[6] 葛会奇,贾天柱.HPLC测定五味子的最佳采收期及五味子醇甲在果实中的分布[J].中成药,2007,29(6):862-864.
[7] 宋九华,杨孝容.HPLC测定安神补心丸中五味子醇甲、五味子酯甲和五味子甲素的含量[J].中成药,2008,30(3):380-383.

备注/Memo

备注/Memo:
收稿日期:2016-05-30
基金项目:贵州省“高层次创新型人才培养”项目(市科合人才〔2015〕35号)
作者简介:张强(1979-),男,主管中药师,理学学士,主要从事药品检验及新药研究工作。
通信作者:冯华(1977-),男,副主任药师,理学硕士,主要从事药品质量标准及新药研究工作。E-mail:fenghua781014@163.com
更新日期/Last Update: 2017-07-11