[1]马正民,颜春鲁,刘永琦,等.敦煌医方四时常服方对镉染毒大鼠肾氧化应激的影响[J].甘肃中医药大学学报,2018,35(01):5-9.[doi:10.16841/j.issn1003-8450.2018.01.02]
 MA Zhengmin,YAN Chunlu,LIU Yongqi,et al.Dunhuang Sishi Changfu Fang’s effect on renal oxidative stress in rats infected by cadmium[J].Journal of Gansu University of Chinese Medicine,2018,35(01):5-9.[doi:10.16841/j.issn1003-8450.2018.01.02]
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敦煌医方四时常服方对镉染毒大鼠肾氧化应激的影响()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
35卷
期数:
2018年01期
页码:
5-9
栏目:
论著
出版日期:
2018-02-25

文章信息/Info

Title:
Dunhuang Sishi Changfu Fang’s effect on renal oxidative stress in rats infected by cadmium
作者:
马正民1 颜春鲁2 刘永琦3 宋敏1 陈正君4
1. 甘肃中医药大学中医临床学院, 甘肃 兰州 730000;
2. 甘肃中医药大学教学实验实训中心, 甘肃 兰州 730000;
3. 甘肃中医药大学基础医学院, 甘肃 兰州 730000;
4. 甘肃中医药大学期刊编辑部, 甘肃 兰州 730000
Author(s):
MA Zhengmin1 YAN Chunlu2 LIU Yongqi3 SONG Min1 CHEN Zhengjun4
1. Clinical College of TCM, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730000, China;
2. Teaching Experiment Training Center, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730000, China;
3. Basic Medicine College, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730000, China;
4. Journal Editorial Department, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730000, China
关键词:
敦煌医方四时常服方镉染毒大鼠肾脏指数超氧化物歧化酶丙二醛谷草转氨酶实验研究
Keywords:
Dunhuang medical prescriptionsSishi Changfu Fangcadmium infectedratsrenal indexSODMDAASTexperimental study
分类号:
R285.5
DOI:
10.16841/j.issn1003-8450.2018.01.02
摘要:
目的 研究敦煌医方四时常服方对镉染毒大鼠肾氧化应激的影响。方法 将60只Wistar大鼠随机分为空白组,模型组,阳性对照组,四时常服方高、中、低剂量组6组,每组10只。空白组腹腔注射0.9%氯化钠注射液,其他5组均腹腔注射0.1%氯化镉溶液1.5 mg/kg,制备镉染毒大鼠模型,每周5次,共5周,染毒的同时分别给予相应药物进行干预,每天1次,连续灌胃5周后处死大鼠,取血和肾组织,检测血清谷草转氨酶(AST)活性及肾组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,并测定肾脏指数。结果 与空白组比较,模型组肾组织中SOD活性明显降低(P<0.01),肾脏指数、肾组织中MDA含量和血清AST活性明显升高(P<0.01);与模型组比较,四时常服方各剂量组肾组织中SOD活性明显升高(P<0.01),肾脏指数、肾组织中MDA含量和血清AST活性均明显降低(P<0.01),其中对SOD,MDA的作用以四时常服方高剂量组最为显著(P<0.05)。结论 敦煌医方四时常服方对镉染毒大鼠肾氧化应激具有一定的拮抗作用,可缓解大鼠染镉中毒后肾脏的损伤。
Abstract:
Objective To investigate the effect of Dunhuang Sishi Changfu Fang on renal oxidative stress in rats infected by cadmium.Methods 60 Wistar rats were randomly divided into 6 groups:blank group,model group,positive control group, the high-,medium-and low-dose group of the medical prescription of Sishi Changfu Fang,10 rats in each. The blank group was intraperitoneally injected with 0.9% NaCl solution,and the remaining five groups were intraperitoneally injected with 0.1% cadmium chloride solution (1.5 mg/kg) for modeling,5 times a week,lasting for 5 weeks. At the same time,the rats exposed to cadmium were given drug intervention for treatment once a day. 5 weeks later,blood and kidney tissues were taken out to detect the contents of aspartate ami-notransferase (AST),superoxide dismutase (SOD) and malondialdehyde (MDA). Renal index was determined.Results Compared with blank group,the activity of SOD significantly decreased (P<0.01),rena index,the content of MDA and the activity of AST increased (P<0.01) in model group,the differences were statistically significant;compared with model group, the activity of SOD increased (P<0.01),renal index,the content of MDA,the activity of AST decreased (P<0.01) in different doses groups of Sishi Changfu Fang,while was most effective on SOD and MDA in the high-dose group (P<0.05).Conclusion The Sishi Changfu Fang has antagonistic effect on oxidative stress in renal function to alleviate the injury of kidney in rats infected by cadmium.

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备注/Memo

备注/Memo:
收稿日期:2017-2-21。
基金项目:甘肃省自然科学基金项目(1508RJZA046);甘肃中医药大学中青年基金项目(ZQ2014-9);敦煌医学与转化省部共建教育部重点实验室开放基金项目(DHYX1213-004);甘肃中医药大学教学研究与教学改革综合项目(ZH-201606)。
作者简介:马正民(1994-),男,2014级中医骨伤在读本科生。
通信作者:颜春鲁(1979-),男,副教授,医学硕士,主要从事中医药防治免疫系统疾病的研究。E-mail:yanchl1979@126.com
更新日期/Last Update: 1900-01-01