[1]郭成龙,何玲,郭小荣,等.生骨再造丸联合髓芯减压术对激素性股骨头缺血性坏死模型兔血脂水平及骨小梁结构的影响[J].甘肃中医药大学学报,2018,35(01):18-22.[doi:10.16841/j.issn1003-8450.2018.01.05]
 GUO Chenglong,HE Ling,GUO Xiaorong,et al.Effect of Shenggu Zaizao Wan combined with myeloid drilling core decompression in treating steroid induced avascular necrosis of the femoral head on blood lipid level and trabecular architecture in model rabbit[J].Journal of Gansu University of Chinese Medicine,2018,35(01):18-22.[doi:10.16841/j.issn1003-8450.2018.01.05]
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生骨再造丸联合髓芯减压术对激素性股骨头缺血性坏死模型兔血脂水平及骨小梁结构的影响()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
35卷
期数:
2018年01期
页码:
18-22
栏目:
实验研究
出版日期:
2018-02-25

文章信息/Info

Title:
Effect of Shenggu Zaizao Wan combined with myeloid drilling core decompression in treating steroid induced avascular necrosis of the femoral head on blood lipid level and trabecular architecture in model rabbit
作者:
郭成龙1 何玲2 郭小荣1 曹林忠2 杨小锋2 张虎林1 张晓刚1
1. 甘肃中医药大学附属医院脊柱外科, 甘肃 兰州 730020;
2. 甘肃中医药大学中医临床学院, 甘肃 兰州 730000
Author(s):
GUO Chenglong1 HE Ling2 GUO Xiaorong1 CAO Linzhong2 YANG Xiaofeng2 ZHANG Hulin1 ZHANG Xiaogang1
1. Spine Surgical Department, Affiliated Hospital to Gansu University of Chinese Medicine, Lanzhou, Gansu, 730020, China;
2. Clinical College of TCM, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730000, China
关键词:
激素性股骨头缺血性坏死生骨再造丸髓芯减压术实验研究
Keywords:
steroid femoral head necrosisrabbitShenggu Zaizao Wanmyeloid drilling core decompositionexperimental study
分类号:
R285.5
DOI:
10.16841/j.issn1003-8450.2018.01.05
摘要:
目的 观察生骨再造丸联合髓芯减压术对激素性股骨头缺血性坏死模型兔血脂水平及骨小梁结构的影响,探讨生骨再造丸联合髓芯减压术治疗激素性股骨头缺血性坏死的作用机制。方法 将60只雄性28周龄青紫蓝兔随机分为空白组、模型组、生骨再造丸组、手术组、综合治疗组5组,其中空白组组8只,其余4组各13只。除空白组外,其余4组通过耳缘静脉注射大肠杆菌内毒素(LPS)及臀肌注射甲基强的松龙(MP)进行造模。造模成功后,生骨再造丸组以4.6 mL/(kg·d)剂量灌胃生骨再造丸浓缩液,手术组给予髓芯减压术,综合治疗组在髓芯减压术的基础上灌胃生骨再造丸浓缩液,空白组及模型组给予等量生理盐水灌胃。干预8周后,观察各组兔一般情况,检测并比较各组血清总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平,取股骨头标本切片,行HE染色,观察并比较各组兔骨小梁结构及密度、骨细胞形态、髓腔脂肪细胞数目。结果 模型组骨小梁排列散乱、稀疏,部分骨小梁萎缩、断裂,周围无成骨细胞,骨髓内造血细胞和脂肪细胞坏死,骨髓腔扩大;生骨再造丸组、手术组、综合治疗组骨小梁均较模型组密集,脂肪细胞逐次减少,造血细胞增多,有利于骨坏死的修复,综合治疗组尤为明显。与空白组比较,模型组血清TG,TC,LDL-C水平明显升高(P<0.01),HDL-C水平明显降低(P<0.01);与模型组比较,生骨再造丸组和综合治疗组血清TG,TC,LDL-C水平明显降低,HDL-C水平明显升高,差异均有统计学意义(P<0.05或P<0.01),且综合治疗组对TC,LDL-C的改善作用更显著(P<0.05)。结论 生骨再造丸联合髓芯减压术可降低激素性股骨头缺血性坏死模型兔血脂,并增加股骨头骨小梁密度。
Abstract:
Objective To investigate the therapeutic effect of co-administration of Shenggu Zaizao Wan and core decomposition on blood lipid level and trabecular architecture in model rabbits with steroid induced avascular necrosis of the femoral head (SANFH),as well as to explore the underlying mechanism.Methods A total of 60 28-week-old male chinchilla rabbits were randomly divided into control group of 8 rabbits and experimental group of 52 rabbits. The control group (group A) was left untreated. For experimental group,lipopolysaccharide and methylprednisolone were co-administered incessantly for 4 weeks to model steroid induced avascular necrosis of the femoral head. After modeling,experimental group was immediately subdivided into 4 subgroups with 13 rabbits in each. They were as follows:the model control group (group B),took no further treatment;the Shenggu Zaizao Wan group (group C),was continuously treated with Shenggu Zaizao Wan concentrate with a dose of 4.6 mL/(kg·d);the surgery group (group D),was intervened with myeloid drilling core decomposition;and the comprehensive treatment group (group E),was treated by Shenggu Zaizao Wan concentrate with a dose of 4.6 mL/(kg·d) after core decomposition. After 8 weeks,blood samples were collected for blood lipid measurement (TC,TG,HDL-C,LDL-C),then all rabbits were sacrificed and caput femorises were removed for HE staining. Trabecular architecture and density,osteocyte morphology,the number of fat cell in the medullary cavity were observed and compared among all groups.Results HE staining of caput femoris showed that there were necrotic niduses in group B,while there were denser trabeculae,gradual decrease of fat cells,increase of hematopoietic cells,restoration of osteonecrosis in the other modeling groups,especially distinct in group E. Compared with group A,blood lipid profiles changed in the other model groups,TG,TC,LDL-C increased (P<0.01),HDL-C decreased (P<0.01),especially in group B. Compared with group B,blood lipid profiles were ameliorated in group C,D and E,while TG,TC,LDL-C decreased,HDL-C increased. The difference was statistically significant (P<0.05 or P<0.01),especially in group E (P<0.05).Conclusion The combined therapy is effective in reducing blood fat cells and improving bone mineral density in model rabbits with SANFH.

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备注/Memo

备注/Memo:
收稿日期:2017-3-12。
基金项目:兰州市科技局2014立项课题(2014-1-25)。
作者简介:郭成龙(1979-),男,副主任医师,医学硕士,主要从事颈肩腰腿痛、骨与关节病的中医药防治及研究工作。
通信作者:何玲(1978-),女,主治医师,医学硕士,主要从事中西医结合治疗常见肿瘤及相关病理研究工作。E-mail:107228466@qq.com
更新日期/Last Update: 1900-01-01